European Eel Sperm Diluent for Short‐term Storage
Identifieur interne : 000460 ( Main/Exploration ); précédent : 000459; suivant : 000461European Eel Sperm Diluent for Short‐term Storage
Auteurs : Ds Pe Aranda ; L. Pérez ; V. Gallego ; R. Barrera [Espagne] ; M. Jover ; Jf AsturianoSource :
- Reproduction in Domestic Animals [ 0936-6768 ] ; 2010-06.
Abstract
The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining over 3 weeks’ conservation times under refrigeration, but they rendered bad results in the European species. In the present study, several diluents were developed taking as basis the P1 medium, and using different dilution ratios (1 : 50, 1 : 100) and two pH (6.5, 8.5). The effect of the addition of bovine serum albumin (BSA, 2% w/v) was also evaluated. At 24 h, undiluted samples already showed significant lower motility and viability than sperm samples diluted in the different media. The results for diluents with pH 6.5 and 8.5 were different. Spermatozoa diluted in media at pH 6.5 cannot be activated at 24 h, while samples diluted in the diluents with pH 8.5 and added with BSA did not show significant differences with respect to the fresh sperm motility until 48 h. The viability (percentage of alive cells) did not show differences until 1 week, independent of the dilution ratio. After 1 week, the motility was approximately 30% in the media containing BSA, which presented no differences for head size of the spermatozoa (perimeter and area) until 72 h and 1 week, respectively. In conclusion, the combination of one medium having similar physico‐chemical characteristics to the seminal plasma, including pH 8.5, and supplemented with BSA can be used in different dilution ratios for the sperm’s short‐term storage, preserving its motility capacity.
Url:
DOI: 10.1111/j.1439-0531.2008.01206.x
Affiliations:
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Jover</name></author><author><name sortKey="Asturiano, Jf" sort="Asturiano, Jf" uniqKey="Asturiano J" first="Jf" last="Asturiano">Jf Asturiano</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7342A7152D732437342047307835C432E3944CF6</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1111/j.1439-0531.2008.01206.x</idno><idno type="url">https://api.istex.fr/document/7342A7152D732437342047307835C432E3944CF6/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C15</idno><idno type="wicri:Area/Istex/Curation">000C15</idno><idno type="wicri:Area/Istex/Checkpoint">000197</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000197</idno><idno type="wicri:doubleKey">0936-6768:2010:Pe Aranda D:european:eel:sperm</idno><idno type="wicri:Area/Main/Merge">000465</idno><idno type="wicri:Area/Main/Curation">000460</idno><idno type="wicri:Area/Main/Exploration">000460</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">European Eel Sperm Diluent for Short‐term Storage</title><author><name sortKey="Pe Aranda, Ds" sort="Pe Aranda, Ds" uniqKey="Pe Aranda D" first="Ds" last="Pe Aranda">Ds Pe Aranda</name><affiliation><wicri:noCountry code="subField">Valencia</wicri:noCountry></affiliation></author><author><name sortKey="Perez, L" sort="Perez, L" uniqKey="Perez L" first="L" last="Pérez">L. 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Jover</name><affiliation><wicri:noCountry code="subField">Valencia</wicri:noCountry></affiliation></author><author><name sortKey="Asturiano, Jf" sort="Asturiano, Jf" uniqKey="Asturiano J" first="Jf" last="Asturiano">Jf Asturiano</name><affiliation><wicri:noCountry code="subField">Valencia</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Reproduction in Domestic Animals</title><idno type="ISSN">0936-6768</idno><idno type="eISSN">1439-0531</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-06">2010-06</date><biblScope unit="volume">45</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="407">407</biblScope><biblScope unit="page" to="415">415</biblScope></imprint><idno type="ISSN">0936-6768</idno></series><idno type="istex">7342A7152D732437342047307835C432E3944CF6</idno><idno type="DOI">10.1111/j.1439-0531.2008.01206.x</idno><idno type="ArticleID">RDA1206</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0936-6768</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The sperm of European eel shows a high density and the time of spermatozoa motility is very short after activation with sea water. These characteristics make difficult the sperm handling and its quality assessment. Several diluents were previously described for the Japanese eel obtaining over 3 weeks’ conservation times under refrigeration, but they rendered bad results in the European species. In the present study, several diluents were developed taking as basis the P1 medium, and using different dilution ratios (1 : 50, 1 : 100) and two pH (6.5, 8.5). The effect of the addition of bovine serum albumin (BSA, 2% w/v) was also evaluated. At 24 h, undiluted samples already showed significant lower motility and viability than sperm samples diluted in the different media. The results for diluents with pH 6.5 and 8.5 were different. Spermatozoa diluted in media at pH 6.5 cannot be activated at 24 h, while samples diluted in the diluents with pH 8.5 and added with BSA did not show significant differences with respect to the fresh sperm motility until 48 h. The viability (percentage of alive cells) did not show differences until 1 week, independent of the dilution ratio. After 1 week, the motility was approximately 30% in the media containing BSA, which presented no differences for head size of the spermatozoa (perimeter and area) until 72 h and 1 week, respectively. In conclusion, the combination of one medium having similar physico‐chemical characteristics to the seminal plasma, including pH 8.5, and supplemented with BSA can be used in different dilution ratios for the sperm’s short‐term storage, preserving its motility capacity.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Asturiano, Jf" sort="Asturiano, Jf" uniqKey="Asturiano J" first="Jf" last="Asturiano">Jf Asturiano</name><name sortKey="Gallego, V" sort="Gallego, V" uniqKey="Gallego V" first="V" last="Gallego">V. Gallego</name><name sortKey="Jover, M" sort="Jover, M" uniqKey="Jover M" first="M" last="Jover">M. Jover</name><name sortKey="Pe Aranda, Ds" sort="Pe Aranda, Ds" uniqKey="Pe Aranda D" first="Ds" last="Pe Aranda">Ds Pe Aranda</name><name sortKey="Perez, L" sort="Perez, L" uniqKey="Perez L" first="L" last="Pérez">L. Pérez</name></noCountry><country name="Espagne"><noRegion><name sortKey="Barrera, R" sort="Barrera, R" uniqKey="Barrera R" first="R" last="Barrera">R. Barrera</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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